SIRT1 mouse mAb

    • 货号:YM1416
    • 应用:WB;IP
    • 种属:Human
      • 靶点:
      • SIRT1
      • 简介:
      • >>Nicotinate and nicotinamide metabolism;>>Metabolic pathways;>>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Glucagon signaling pathway;>>Alcoholic liver disease;>>Amphetamine addiction;>>MicroRNAs in cancer
      • 基因名称:
      • sirt1
      • Human Swiss Prot No:
      • Q96EB6
      • Mouse Swiss Prot No:
      • Q923E4
      • 免疫原:
      • Purified recombinant human SIRT1 protein fragments expressed in E.coli.
      • 特异性:
      • This antibody detects endogenous levels of SIRT1 and does not cross-react with related proteins.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • wb dilution 1:1000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • 75SirT1;BA57G10.4;BA57G10.4;hSIR2;hSIRT1;HST2, S. cerevisiae, homolog of;NAD dependent deacetylase SIRT1;NAD dependent deacetylase sirtuin 1;NAD dependent protein deacetylase sirtuin 1;OTTHUMP00000198111;OTTHUMP00000198112;Regulatory protein SIR2 homolog 1;SIR1_HUMAN;SIR2 like 1;SIR2 like protein 1;SIR2, S.cerevisiae, homolog-like 1;SIR2-like protein 1;SIR2ALPHA;SIR2ALPHA;SIR2alpha protein;SIR2alpha protein;SIR2L1;SIRT 1;Sirt1;SIRT1 Sir2 like proteins (siruitins) type 1;SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);SirtT1 75 kDa fragment;sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae);Sirtuin 1;sirtuin;Sirtuin type 1;Sirtuin type 1.
      • 实测条带:
      • 85-110kD
      • 背景:
      • This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
      • 功能:
      • catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity.,function:NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1
      • 细胞定位:
      • Nucleus, PML body . Cytoplasm . Nucleus . Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491). Colocalized with APEX1 in the nucleus (PubMed:19934257). May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825). Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178). .; [SirtT1 75 kDa fragment]: Cytoplasm . Mitochondrion .
      • 组织表达:
      • Widely expressed.

      CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell
      货号:YM1416

      • 产品图片
      • Western blot detection of SIRT1 in Hela,Jurkat,Ramos,A549 and CEM cell lysates using SIRT1 mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.
      • Immunoprecipitation analysis of Hela cell lysates using SIRT1 mouse mAb.