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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CHD3(C-term) mouse mAb
- 货号:YM1248
- 应用:WB;IHC;FCM
- 种属:Human
- 免疫原:
- Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- wb 1:1000 1:500-1:1000 fcm 1:100
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha;Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
- 背景:
- This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
- 功能:
- disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
- 细胞定位:
- Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
- Western blot detection of CHD3 (C-terminus) in Y79 and Raji cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 226KDa.Observed band size: 260KDa.
- IHC of paraffin-embedded human colon using anti-CHD3 (C-terminus) diluted 1/500-1/1000.
- Flow Cytometry analysis of K562 cells stained with CHD3 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).