MEK Kinase-2 Monoclonal Antibody
- 货号：YM0434
- 应用：WB;IF;ELISA
- 种属：Human
- 简介：
- >>MAPK signaling pathway;>>Gap junction;>>GnRH signaling pathway
- 蛋白名称：
- Mitogen-activated protein kinase kinase kinase 2
- 免疫原：
- Purified recombinant fragment of human MEK Kinase-2 expressed in E. Coli.
- 特异性：
- MEK Kinase-2 Monoclonal Antibody detects endogenous levels of MEK Kinase-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MAP3K2;MAPKKK2;MEKK2;Mitogen-activated protein kinase kinase kinase 2;MAPK/ERK kinase kinase 2;MEK kinase 2;MEKK 2
- 背景：
- The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-524.,function:Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Upon EGF stimulation, translocates into the nucleus.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes.,
- 细胞定位：
- Cytoplasm . Nucleus . Upon EGF stimulation, translocates into the nucleus.
- 组织表达：
- Brain,Platelet,T-cell,Teratocarcinoma,Whole embryo,
- Western Blot analysis using MEK Kinase-2 Monoclonal Antibody against HEK293 (1) and MAP3K2-hIgGFc transfected HEK293 (2) cell lysate.
- Immunofluorescence analysis of 3T3-L1 cells using MEK Kinase-2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
