Ku-80 Monoclonal Antibody
- 货号：YM0409
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human;Mouse
- 简介：
- >>Non-homologous end-joining
- 蛋白名称：
- X-ray repair cross-complementing protein 5
- 免疫原：
- Purified recombinant fragment of human Ku-80 expressed in E. Coli.
- 特异性：
- Ku-80 Monoclonal Antibody detects endogenous levels of Ku-80 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- XRCC5;G22P2;X-ray repair cross-complementing protein 5;86 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair pr
- 背景：
- The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],
- 功能：
- developmental stage:Expression increases during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86.,domain:The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of t
- 细胞定位：
- Nucleus . Nucleus, nucleolus . Chromosome .
- 组织表达：
- Cervix carcinoma,Coronary artery,Heart,Neuroblastoma,Osteoblast,Thy
- Western Blot analysis using Ku-80 Monoclonal Antibody against HeLa (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) with DAB staining using Ku-80 Monoclonal Antibody.
- Immunofluorescence analysis of Hela cells using Ku-80 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Hela cells using Ku-80 Monoclonal Antibody (green) and negative control (purple).
