• 靶点：
• Fyn

• 简介：
• >>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Axon guidance;>>Osteoclast differentiation;>>Focal adhesion;>>Adherens junction;>>Platelet activation;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Cholinergic synapse;>>Prion disease;>>Pathogenic Escherichia coli infection;>>Viral myocarditis

• 基因名称：
• FYN

• 蛋白名称：
• Tyrosine-protein kinase Fyn

• Human Gene Id：
• 2534

• Human Swiss Prot No：
• P06241

• Mouse Swiss Prot No：
• P39688

• 免疫原：
• Purified recombinant fragment of human Fyn expressed in E. Coli.

• 特异性：
• Fyn Monoclonal Antibody detects endogenous levels of Fyn protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• FYN;Tyrosine-protein kinase Fyn;Proto-oncogene Syn;Proto-oncogene c-Fyn;Src-like kinase;SLK;p59-Fyn

• 分子量：
• 61kD

• 背景：
• This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Manganese.,enzyme regulation:Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site.,function:Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1

• 细胞定位：
• Cytoplasm. Nucleus. Cell membrane. Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.

• 组织表达：
• Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.

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