EphA1 Monoclonal Antibody
- 货号:YM0220
- 应用:WB;IHC;IF;ELISA
- 种属:Human
- 蛋白名称:
- Ephrin type-A receptor 1
- 免疫原:
- Purified recombinant fragment of EphA1 expressed in E. Coli.
- 特异性:
- EphA1 Monoclonal Antibody detects endogenous levels of EphA1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EPHA1;EPH;EPHT;EPHT1;Ephrin type-A receptor 1;hEpha1;EPH tyrosine kinase;EPH tyrosine kinase 1;Erythropoietin-producing hepatoma receptor;Tyrosine-protein kinase receptor EPH
- 背景:
- This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,tissue specificity:Overexpressed in several carcinomas.,
- 细胞定位:
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达:
- Overexpressed in several carcinomas.
- Western Blot analysis using EphA1 Monoclonal Antibody against A549 (1) and HeLa (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization with DAB staining using EphA1 Monoclonal Antibody.