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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Emt Monoclonal Antibody
- 货号:YM0218
- 应用:WB;IF;FCM;ELISA
- 种属:Human
- 简介:
- >>Chemokine signaling pathway;>>T cell receptor signaling pathway;>>Leukocyte transendothelial migration
- 蛋白名称:
- Tyrosine-protein kinase ITK/TSK
- 免疫原:
- Purified recombinant fragment of human Emt expressed in E. Coli.
- 特异性:
- Emt Monoclonal Antibody detects endogenous levels of Emt protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ITK;EMT;LYK;Tyrosine-protein kinase ITK/TSK;Interleukin-2-inducible T-cell kinase;IL-2-inducible T-cell kinase;Kinase EMT;T-cell-specific kinase;Tyrosine-protein kinase Lyk
- 背景:
- This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,
- 细胞定位:
- Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
- 组织表达:
- T-cell lines and natural killer cell lines.
- Western Blot analysis using Emt Monoclonal Antibody against Jurkat cell lysate.
- Immunofluorescence analysis of Jurkat cells using Emt Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of Jurkat cells using Emt Monoclonal Antibody (blue) and negative control (red).