Chitotriosidase Monoclonal Antibody
- 货号：YM0152
- 应用：WB;ELISA
- 种属：Human
- 简介：
- >>Amino sugar and nucleotide sugar metabolism;>>Metabolic pathways
- 免疫原：
- Purified recombinant fragment of Chitotriosidase (aa22-137) expressed in E. Coli.
- 特异性：
- Chitotriosidase Monoclonal Antibody detects endogenous levels of Chitotriosidase protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CHIT1;Chitotriosidase-1;Chitinase-1
- 背景：
- Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012],
- 功能：
- catalytic activity:Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.,disease:Very high plasma levels of CHIT1 are found in patients with Gaucher disease type 1 (GD I). This can be used as diagnostic aid and to evaluate the success of treatment. Successful therapy brings the CHIT1 activity levels back to normal.,function:Degrades chitin and chitotriose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.,polymorphism:A 24 bp duplication in exon 10 leads to the activation of an alternative splice site and the production of an inactive protein. About 6% of the population are deficient for CHIT1 activity, while 35% are carriers and show reduced enzyme levels. People with CHIT1 deficiency appear perfectly healthy.,similarity:Belongs to the glycosyl hydrolase 18 family.,similarity:Belongs
- 细胞定位：
- Secreted. Lysosome. A small proportion is lysosomal.
- 组织表达：
- Detected in spleen. Secreted by cultured macrophages.
- Western Blot analysis using Chitotriosidase Monoclonal Antibody against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).
