CD14 Monoclonal Antibody

    • 货号:YM0103
    • 应用:IHC;IF;FCM;ELISA
    • 种属:Human
      • 靶点:
      • CD14
      • 简介:
      • >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Phagosome;>>Toll-like receptor signaling pathway;>>Hematopoietic cell lineage;>>Alcoholic liver disease;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Amoebiasis;>>Tuberculosis;>>Transcriptional misregulation in cancer;>>Acute myeloid leukemia;>>Lipid and atherosclerosis
      • 基因名称:
      • CD14
      • 蛋白名称:
      • Monocyte differentiation antigen CD14
      • Human Gene Id:
      • 929
      • Human Swiss Prot No:
      • P08571
      • Mouse Swiss Prot No:
      • P10810
      • 免疫原:
      • Purified recombinant fragment of human CD14 expressed in E. Coli.
      • 特异性:
      • CD14 Monoclonal Antibody detects endogenous levels of CD14 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200
      • 纯化工艺:
      • Affinity purification
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CD14;Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD antigen CD14
      • 背景:
      • The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010],
      • 功能:
      • function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.,online information:CD14 entry,similarity:Contains 11 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.,tissue specificity:Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.,
      • 细胞定位:
      • Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Membrane raft . Golgi apparatus . Secreted forms may arise by cleavage of the GPI anchor. .
      • 组织表达:
      • Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
      • 产品图片
      • Immunohistochemistry analysis of paraffin-embedded human lymphnode, showing membrane localization with DAB staining using CD14 Monoclonal Antibody.
      • Flow cytometric analysis of human PBMC using CD14 Monoclonal Antibody.