ApoA-IV Monoclonal Antibody
- 货号：YM0032
- 应用：WB;ELISA
- 种属：Human
- 简介：
- >>Fat digestion and absorption;>>Vitamin digestion and absorption;>>Cholesterol metabolism;>>Lipid and atherosclerosis
- 蛋白名称：
- Apolipoprotein A-IV
- 免疫原：
- Purified recombinant fragment of ApoA-IV (aa21-396) expressed in E. Coli.
- 特异性：
- ApoA-IV Monoclonal Antibody detects endogenous levels of ApoA-IV protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- APOA4;Apolipoprotein A-IV;Apo-AIV;ApoA-IV;Apolipoprotein A4
- 背景：
- Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008],
- 功能：
- domain:Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.,function:May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.,online information:The Singapore human mutation and polymorphism database,polymorphism:Eight alleles have been characterized (APOA-IV*0 to APOA-IV*7). APOA-IV*1 is the major allele (90%), APOA-IV*2 is also common (8%), the others are rare alleles.,similarity:Belongs to the apolipoprotein A1/A4/E family.,tissue
- 组织表达：
- Synthesized primarily in the intestine and secreted in plasma.
- Western Blot analysis using ApoA-IV Monoclonal Antibody against truncated APOA4-His recombinant protein (1),truncated APOA4(aa21-396)-hIgGFc transfected CHO-K1 cell lysate(2),human serum (3) and human plasma (4).
