Total XRCC4 Cell-Based Colorimetric ELISA Kit
- 货号：KA4328C
- 应用：ELISA
- 种属：Human
- 其他名称：
- DNA repair protein XRCC4 (X-ray repair cross-complementing protein 4)
- 背景：
- function:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,PTM:Monoubiquitinated.,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.,similarity:Belongs to the XRCC4 family.,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF.,tissue specificity:Widely expressed.,
- 功能：
- non-recombinational repair, in utero embryonic development, cell activation, somatic diversification of immune receptors, somatic recombination of immunoglobulin genes during immune response, somatic diversification of immunoglobulins during immune response, hemopoietic progenitor cell differentiation, adaptive immune response,immune effector process, lymphoid progenitor cell differentiation, pro-B cell differentiation, immunoglobulin production,immunoglobulin production during immune response, production of molecular mediator of immune response, leukocyte mediated immunity, lymphocyte mediated immunity, adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains, immune system development, leukocyte differentiation, somatic diversification of immune receptors via germline recombination within a single locus, DNA metabolic pro
- 细胞定位：
- Nucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4, C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3). .
