XRCC4 Polyclonal Antibody

    • 货号:YT4921
    • 应用:WB;ELISA
    • 种属:Human;Rat;Mouse;
      • 靶点:
      • XRCC4
      • 简介:
      • >>Non-homologous end-joining
      • 基因名称:
      • XRCC4
      • 蛋白名称:
      • DNA repair protein XRCC4
      • Human Gene Id:
      • 7518
      • Human Swiss Prot No:
      • Q13426
      • Mouse Swiss Prot No:
      • Q924T3
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human XRCC4. AA range:261-310
      • 特异性:
      • XRCC4 Polyclonal Antibody detects endogenous levels of XRCC4 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • XRCC4;DNA repair protein XRCC4;X-ray repair cross-complementing protein 4
      • 实测条带:
      • 40kD
      • 背景:
      • The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],
      • 功能:
      • function:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,PTM:Monoubiquitinated.,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.,similarity:Belongs to the XRCC4 family.,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dep
      • 细胞定位:
      • Nucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4, C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3). .
      • 组织表达:
      • Widely expressed.
      • 产品图片
      • Western Blot analysis of various cells using XRCC4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of lysates from COLO205 cells, using XRCC4 Antibody. The lane on the right is blocked with the synthesized peptide.