Catalog: YP1072
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
AIP4 Phospho Tyr420
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
IHC, IF, ELISA
MW
103kD (Calculated)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FIyGN
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
103kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435
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Specificity:
Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FIyGN
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Gene Name:
ITCH
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Protein Name:
E3 ubiquitin-protein ligase Itchy homolog
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Other Name:
ITCH ;
E3 ubiquitin-protein ligase Itchy homolog ;
Itch ;
Atrophin-1-interacting protein 4 ;
AIP4 ;
NFE2-associated polypeptide 1 ;
NAPP1
E3 ubiquitin-protein ligase Itchy homolog ;
Itch ;
Atrophin-1-interacting protein 4 ;
AIP4 ;
NFE2-associated polypeptide 1 ;
NAPP1
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Background:
itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],
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Function:
Function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 C2 domain.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 4 WW domains.,subunit:Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts with Epstein-Barr virus LMP2A. Interacts with NOTCH1, OCLN, JUN and JUNB. Interacts with NDFIP1 in vitro (By similarity). Interacts with ARHGEF7.,tissue specificity:Widely expressed.,
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Cellular Localization:
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm . Nucleus . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . May be recruited to exosomes by NDFIP1 (PubMed:18819914). Localizes to plasma membrane upon CXCL12 stimulation where it co-localizes with CXCL4 (PubMed:14602072). Localization to early endosomes is increased upon CXCL12 stimulation where it co-localizes with DTX3L and CXCL4 (PubMed:24790097). .
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Tissue Expression:
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Research Areas:
>>Ubiquitin mediated proteolysis ;
>>Endocytosis ;
>>TNF signaling pathway ;
>>Non-alcoholic fatty liver disease
>>Endocytosis ;
>>TNF signaling pathway ;
>>Non-alcoholic fatty liver disease
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP1072
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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