The antiserum was produced against synthesized peptide derived from human ICK around the phosphorylation site of Tyr159. AA range:125-174

Phospho-Intestinal Cell Kinase (Y159) Polyclonal Antibody detects endogenous levels of Intestinal Cell Kinase protein only when phosphorylated at Y159.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TDyVS

ICK

Serine/threonine-protein kinase ICK

ICK ;
KIAA0936 ;
Serine/threonine-protein kinase ICK ;
Intestinal cell kinase ;
hICK ;
Laryngeal cancer kinase 2 ;
LCK2 ;
MAK-related kinase ;
MRK

Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008],

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Disease:Defects in ICK are the cause of endocrine-cerebroosteodysplasia (ECO) [MIM:612651]. ECO is a previously unidentified neonatal lethal recessive disorder with multiple anomalies involving the endocrine, cerebral, and skeletal systems.,Function:May play a key role in the development of multiple organ systems and particularly in cardiac development.,PTM:Autophosphorylated on serine and threonine residues. May play a role in enzyme activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360). Cytosolic localization was shown in rat embryonic cardiomyocytes by immunostaining (PubMed:8570168).,tissue specificity:Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.,

Nucleus . Cytoplasm, cytosol . Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Also found at the ciliary tip (PubMed:24797473). Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360, PubMed:19185282). .; [Isoform 2]: Cytoplasm . Predominant cytoplasmic localization has been observed with a N-terminally GFP-tagged construct. .

Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.