Catalog: YP0280
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Collected
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Main Information
Target
eIF4B
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ELISA
MW
80kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TGsES
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
80kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437
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Specificity:
Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TGsES
show all
Gene Name:
EIF4B
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Protein Name:
Eukaryotic translation initiation factor 4B
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Other Name:
EIF4B ;
Eukaryotic translation initiation factor 4B ;
eIF-4B
Eukaryotic translation initiation factor 4B ;
eIF-4B
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Background:
function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
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Function:
Function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
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Cellular Localization:
cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body,
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Tissue Expression:
Adipose tissue,Brain,Cervix carcinoma,Epithelium,Liver,Lung
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Research Areas:
>>mTOR signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>Proteoglycans in cancer
>>PI3K-Akt signaling pathway ;
>>Proteoglycans in cancer
show all
Signaling Pathway
Cellular Processes >> Cell growth and death >> Necroptosis
Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells
Organismal Systems >> Immune system >> Toll-like receptor signaling pathway
Organismal Systems >> Immune system >> NOD-like receptor signaling pathway
Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation
Organismal Systems >> Immune system >> Th17 cell differentiation
Organismal Systems >> Development and regeneration >> Osteoclast differentiation
Human Diseases >> Cancer: overview >> Pathways in cancer
Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer
Human Diseases >> Cancer: specific types >> Pancreatic cancer
Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Reference Citation({{totalcount}})
Catalog: YP0280
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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