The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174

Phospho-Mcl-1 (S159) Polyclonal Antibody detects endogenous levels of Mcl-1 protein only when phosphorylated at S159.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DGsLP

MCL1

Induced myeloid leukemia cell differentiation protein Mcl-1

MCL1 ;
BCL2L3 ;
Induced myeloid leukemia cell differentiation protein Mcl-1 ;
Bcl-2-like protein 3 ;
Bcl2-L-3 ;
Bcl-2-related protein EAT/mcl1 ;
mcl1/EAT

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],

Function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.,similarity:Belongs to the Bcl-2 family.,subcellular location:Cytoplasmic, associated with mitochondria.,subunit:Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptototic BCL2-related proteins.,

Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.

>>PI3K-Akt signaling pathway ;
>>Apoptosis ;
>>JAK-STAT signaling pathway ;
>>MicroRNAs in cancer