Catalog: YP0894
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
Splicing factor 1 Phospho Ser82
Host Species
Rabbit
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC, IF, ELISA
MW
68kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SPsPE
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
68kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
Related Products
Primary Antibodies
Splicing factor 1 Rabbit pAb
YT4387
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Primary Antibodies
Splicing factor 1 (Phospho Ser82) Rabbit pAb
YP0894
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ELISA Kits
Total SF1 Cell-Based Colorimetric ELISA Kit
KA4277C
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ELISA Kits
SF1 (Phospho Ser82) Cell-Based Colorimetric ELISA Kit
KA1334C
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human SF1 around the phosphorylation site of Ser82. AA range:48-97
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Specificity:
Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SPsPE
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Gene Name:
SF1
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Protein Name:
Splicing factor 1
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Other Name:
SF1 ;
ZFM1 ;
ZNF162 ;
Splicing factor 1 ;
Mammalian branch point-binding protein ;
BBP ;
mBBP ;
Transcription factor ZFM1 ;
Zinc finger gene in MEN1 locus ;
Zinc finger protein 162
ZFM1 ;
ZNF162 ;
Splicing factor 1 ;
Mammalian branch point-binding protein ;
BBP ;
mBBP ;
Transcription factor ZFM1 ;
Zinc finger gene in MEN1 locus ;
Zinc finger protein 162
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Background:
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016],
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Function:
Alternative products:Additional isoforms seem to exist,Function:Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.,PTM:Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.,similarity:Belongs to the BBP/SF1 family.,similarity:Contains 1 CCHC-type zinc finger.,similarity:Contains 1 KH domain.,subunit:Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15.,tissue specificity:Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.,
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Cellular Localization:
Nucleus.
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Reference Citation({{totalcount}})
Catalog: YP0894
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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