Catalog: YP0743
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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Main Information
Target
RPA2 Phospho Thr21
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ELISA
MW
35kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GYtQS
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
35kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59
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Specificity:
Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GYtQS
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Gene Name:
RPA2
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Protein Name:
Replication protein A 32 kDa subunit
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Other Name:
RPA2 ;
REPA2 ;
RPA32 ;
RPA34 ;
Replication protein A 32 kDa subunit ;
RP-A p32 ;
Replication factor A protein 2 ;
RF-A protein 2 ;
Replication protein A 34 kDa subunit ;
RP-A p34
REPA2 ;
RPA32 ;
RPA34 ;
Replication protein A 32 kDa subunit ;
RP-A p32 ;
Replication factor A protein 2 ;
RF-A protein 2 ;
Replication protein A 34 kDa subunit ;
RP-A p34
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Background:
function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,
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Function:
Function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,
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Cellular Localization:
Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .
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Tissue Expression:
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Research Areas:
>>DNA replication ;
>>Nucleotide excision repair ;
>>Mismatch repair ;
>>Homologous recombination ;
>>Fanconi anemia pathway
>>Nucleotide excision repair ;
>>Mismatch repair ;
>>Homologous recombination ;
>>Fanconi anemia pathway
show all
Reference Citation({{totalcount}})
Catalog: YP0743
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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