IRE1α(Phospho Ser724) (PT1195R) PT™ Rabbit mAb
-YM9004
Catalog: YM9004
Size
Price
Status
Qty.
200μL
$600.00
3 weeks
0
100μL
$340.00
3 weeks
0
40μL
$190.00
3 weeks
0
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Collected
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Main Information
Target
IRE1α Phospho Ser724
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP, ELISA
MW
110kD (Calculated)
110kD (Observed)
Conjugate/Modification
phosphate
Detailed Information
Recommended Dilution Ratio
IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Specificity
IRE1α(Phospho Ser724) Antibody detects endogenous levels of IRE1 protein only when phosphorylated at S724.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RHsFS
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
MW(Calculated)
110kD
MW(Observed)
110kD
Modification
phosphate
Clonality
Monoclonal
Clone Number
PT1195R
Isotype
IgG,Kappa
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Antigen&Target Information
Immunogen:
The specific immunogen used to produce this antibody is proprietary information.
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Specificity:
IRE1α(Phospho Ser724) Antibody detects endogenous levels of IRE1 protein only when phosphorylated at S724.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RHsFS
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Gene Name:
ERN1 IRE1
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Protein Name:
Serine/threonine-protein kinase/endoribonuclease IRE1
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Other Name:
ERN1 ;
IRE1 ;
Serine/threonine-protein kinase/endoribonuclease IRE1 ;
Endoplasmic reticulum-to-nucleus signaling 1 ;
Inositol-requiring protein 1 ;
hIRE1p ;
Ire1-alpha ;
IRE1a
IRE1 ;
Serine/threonine-protein kinase/endoribonuclease IRE1 ;
Endoplasmic reticulum-to-nucleus signaling 1 ;
Inositol-requiring protein 1 ;
hIRE1p ;
Ire1-alpha ;
IRE1a
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Background:
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008],
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Function:
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,Function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue.,
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Cellular Localization:
Endoplasmic reticulum membrane
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Tissue Expression:
Research Areas:
>>Autophagy - animal ;
>>Protein processing in endoplasmic reticulum ;
>>Apoptosis ;
>>Non-alcoholic fatty liver disease ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Lipid and atherosclerosis
>>Protein processing in endoplasmic reticulum ;
>>Apoptosis ;
>>Non-alcoholic fatty liver disease ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Lipid and atherosclerosis
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Signaling Pathway
Cellular Processes >> Transport and catabolism >> Autophagy - animal
Cellular Processes >> Cell growth and death >> Apoptosis
Human Diseases >> Neurodegenerative disease >> Alzheimer disease
Human Diseases >> Neurodegenerative disease >> Parkinson disease
Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Human Diseases >> Neurodegenerative disease >> Huntington disease
Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia
Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
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Catalog: YM9004
Size
Price
Status
Qty.
200μL
$600.00
3 weeks
0
100μL
$340.00
3 weeks
0
40μL
$190.00
3 weeks
0
Add to cart
Collected
Collect
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