UBF-1 Polyclonal Antibody
- 货号：YT4804
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Nucleolar transcription factor 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human UBF1. AA range:501-550
- 特异性：
- UBF-1 Polyclonal Antibody detects endogenous levels of UBF-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- UBTF;UBF;UBF1;Nucleolar transcription factor 1;Autoantigen NOR-90;Upstream-binding factor 1;UBF-1
- 背景：
- This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011],
- 功能：
- function:Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor SL1. It binds specifically to the upstream control element.,PTM:Phosphorylated and activated by PIK3CA.,similarity:Contains 6 HMG box DNA-binding domains.,subunit:Homodimer. Binds to IRS1 and PIK3CA.,
- 细胞定位：
- Nucleus, nucleolus .
- 组织表达：
- Lymph,Placenta,Testis,
- Western Blot analysis of various cells using UBF-1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of Jurkat cells using UBF-1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using UBF1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa and Jurkat cells, using UBF1 Antibody. The lane on the right is blocked with the synthesized peptide.
