MLK3 (Phospho Thr277+Ser281) rabbit pAb
- 货号:YP1580
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 靶点:
- MLK3
- 简介:
- >>MAPK signaling pathway;>>Non-alcoholic fatty liver disease
- 基因名称:
- MAP3K11 MLK3 PTK1 SPRK
- 蛋白名称:
- MLK3 (Phospho Thr277+Ser281)
- Human Gene Id:
- 4296
- Human Swiss Prot No:
- Q16584
- Mouse Gene Id:
- 26403
- Mouse Swiss Prot No:
- Q80XI6
- Rat Gene Id:
- 309168
- Rat Swiss Prot No:
- Q66HA1
- 免疫原:
- Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281)
- 特异性:
- This antibody detects endogenous levels of Human,Mouse,Rat MLK3 (Phospho Thr277+Ser281)
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase)
- 分子量:
- 93kD
- 背景:
- The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.
- 组织表达:
- Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
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- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
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- FLOW-CYTOMEYRT-PROTOCOL
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- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
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- July 13-2018
- Antibody-FAQs
- 产品图片
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).