VE-Cadherin mouse Monoclonal Antibody(3G8)
- 货号：YM3762
- 应用：IHC;IF
- 种属：Human;Mouse;Rat
- 简介：
- >>Cell adhesion molecules;>>Leukocyte transendothelial migration;>>Fluid shear stress and atherosclerosis
- 蛋白名称：
- Cadherin-5 (7B4 antigen) (Vascular endothelial cadherin) (VE-cadherin) (CD antigen CD144)
- 免疫原：
- Synthetic Peptide of VE-Cadherin
- 特异性：
- The antibody detects endogenous VE-Cadherin protein
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- IHC 1:50-300. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- Cadherin-5 (7B4 antigen;Vascular endothelial cadherin;VE-cadherin;CD antigen CD144)
- 背景：
- This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],
- 功能：
- function:Cadherins are calcium dependent cell adhesion proteins.,function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.,similarity:Contains 5 cadherin domains.,subcellular location:Found at cell-cell boundaries and probably at cell-matrix boundaries.,tissue specificity:Endothelial tissues and brain.,
- 细胞定位：
- Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. .
- 组织表达：
- Endothelial tissues and brain.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
