UBE1L Monoclonal Antibody
- 货号：YM0643
- 应用：WB;ELISA
- 种属：Human
- 简介：
- >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases
- 蛋白名称：
- Ubiquitin-like modifier-activating enzyme 7
- 免疫原：
- Purified recombinant fragment of human UBE1L expressed in E. Coli.
- 特异性：
- UBE1L Monoclonal Antibody detects endogenous levels of UBE1L protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- UBA7;UBE1L;UBE2;Ubiquitin-like modifier-activating enzyme 7;Ubiquitin-activating enzyme 7;D8;Ubiquitin-activating enzyme E1 homolog
- 背景：
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008],
- 功能：
- function:Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.,miscellaneous:There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.,tissue specificity:Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.,
- 细胞定位：
- nucleus,nucleoplasm,cytosol,
- 组织表达：
- Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
- Western Blot analysis using UBE1L Monoclonal Antibody against Raji (1) and THP-1 (2) cell lysate.
