DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)

    • Catalog No.:YM3808
    • Applications:WB;ELISA;IP;IF;CoIP
    • Reactivity:Species independent
      • Purity:
      • >95% SDS-PAGE
      • Target:
      • Flag-tag
      • Gene Name:
      • Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
      • Immunogen:
      • Synthetic Peptide of Flag-Tag
      • Specificity:
      • This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse VH IgG1 and VL Kappa was expressed from 293F cells
      • Dilution:
      • WB 1:10000-50000 ELISA 1:20000-50000 IP 1:50-1:200 IF 1:1000 -3000
      • Purification:
      • Protein A purified
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Background:
      • The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

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      • Products Images
      • RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB,IP Human 1:5000 HEK 293 T cell,KYSE30 cell,KYSE450 cell
      • Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
      • Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody(catalog#:RS0001) was diluted at 1:10000
      • 1) Input: 293T transfected N-flag protein Lysate 2) IP product: IP dilute 1: 200, 293T transfected N-flag protein Lysate Western blot analysis: primary antibody : 1:10000 Secondary antibody: Goat anti-Mouse IgG(RS0001), 1: 5000
      • Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody(red YM3808) was diluted at 1:1000(4°C overnight), GFP primary Antibody(green YN5582) was diluted at 1:1000(4°C overnight),