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EPAS-1 Rabbit pAb

-YT5325

Main Information
Target

EPAS-1

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IF, WB, IHC, ELISA

MW

96kD (Calculated)

110-120kD (Observed)

Conjugate/Modification


Unmodified

Detailed Information
Recommended Dilution Ratio
IF 1:50-200; WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; Not yet tested in other applications;
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
96kD
MW(Observed)
110-120kD
Modification
Unmodified
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
Synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385.
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Specificity:
EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
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Gene Name:
EPAS1 BHLHE73 HIF2A MOP2 PASD2
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Protein Name:
Endothelial PAS domain-containing protein 1
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Other Name:
EPAS1 ;
BHLHE73 ;
HIF2A ;
MOP2 ;
PASD2 ;
Endothelial PAS domain-containing protein 1 ;
EPAS-1 ;
Basic-helix-loop-helix-PAS protein MOP2 ;
Class E basic helix-loop-helix protein 73 ;
bHLHe73 ;
HIF-1-alpha-like factor ;
HLF ;
Hypoxia-inducible factor 2-alpha ;
HIF-2-alpha ;
HIF2-alpha ;
Member of PAS protein 2 ;
PAS domain-containing protein 2
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Database Link:
Organism Gene ID SwissProt
Human 2034; Q99814;
Mouse 13819; P97481;
Rat 29452; Q9JHS1;
Background:
endothelial PAS domain protein 1(EPAS1) Homo sapiens This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009],
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Function:
Disease:Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.,Function:Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.,PTM:In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.,PTM:In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.,PTM:Phosphorylated on multiple sites in the CTAD.,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP.,tissue specificity:Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.,
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Cellular Localization:
Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
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Tissue Expression:
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
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Research Areas:
>>Pathways in cancer ;
>>Renal cell carcinoma
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Catalog: YT5325
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200μL
$450.00
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100μL
$280.00
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40μL
$150.00
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