Catalog: YP0754
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
IRP-1 Phospho Ser711
Host Species
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ELISA
MW
85kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-IRP-1 (S711) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S711.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YGsRR
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
85kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711. AA range:681-730
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Specificity:
Phospho-IRP-1 (S711) Polyclonal Antibody detects endogenous levels of IRP-1 protein only when phosphorylated at S711.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YGsRR
show all
Gene Name:
ACO1
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Protein Name:
Cytoplasmic aconitate hydratase
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Other Name:
ACO1 ;
IREB1 ;
Cytoplasmic aconitate hydratase ;
Aconitase ;
Citrate hydro-lyase ;
Ferritin repressor protein ;
Iron regulatory protein 1 ;
IRP1 ;
Iron-responsive element-binding protein 1 ;
IRE-BP 1
IREB1 ;
Cytoplasmic aconitate hydratase ;
Aconitase ;
Citrate hydro-lyase ;
Ferritin repressor protein ;
Iron regulatory protein 1 ;
IRP1 ;
Iron-responsive element-binding protein 1 ;
IRE-BP 1
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Background:
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct
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Function:
Catalytic activity:Citrate = isocitrate.,cofactor:Binds 1 4Fe-4S cluster per subunit.,Function:Binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. This protein also expresses aconitase activity.,online information:Aconitase entry,similarity:Belongs to the aconitase/IPM isomerase family.,
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Cellular Localization:
Cytoplasm, cytosol .
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Tissue Expression:
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Research Areas:
>>Citrate cycle (TCA cycle) ;
>>Glyoxylate and dicarboxylate metabolism ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>2-Oxocarboxylic acid metabolism ;
>>Biosynthesis of amino acids
>>Glyoxylate and dicarboxylate metabolism ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>2-Oxocarboxylic acid metabolism ;
>>Biosynthesis of amino acids
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Reference Citation({{totalcount}})
Catalog: YP0754
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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