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Main Information
Target
MCM4
Host Species
Rabbit
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC, IF, ELISA
MW
85kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PTsPG
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
85kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69
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Specificity:
Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PTsPG
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Gene Name:
MCM4
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Protein Name:
DNA replication licensing factor MCM4
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Other Name:
MCM4 ;
CDC21 ;
DNA replication licensing factor MCM4 ;
CDC21 homolog ;
P1-CDC21
CDC21 ;
DNA replication licensing factor MCM4 ;
CDC21 homolog ;
P1-CDC21
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Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri
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Function:
Function:Involved in the control of DNA replication.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
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Cellular Localization:
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
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Tissue Expression:
Cervix,Epithelium,Testis,
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Research Areas:
>>DNA replication ;
>>Cell cycle
>>Cell cycle
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Signaling Pathway
Catalog: YP0354
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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