The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215

Phospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at T200.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):MKtVC

CAMK4

Calcium/calmodulin-dependent protein kinase type IV

CAMK4 ;
CAMK ;
CAMK-GR ;
CAMKIV ;
Calcium/calmodulin-dependent protein kinase type IV ;
CaMK IV ;
CaM kinase-GR

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008],

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A.,Function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.,PTM:Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.,subunit:Monomer (By similarity). Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca(2+)/calmodulin.,tissue specificity:Expressed in epithelial ovarian cancer tissue.,

Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity). .

>>Calcium signaling pathway ;
>>cAMP signaling pathway ;
>>Longevity regulating pathway ;
>>Apelin signaling pathway ;
>>Osteoclast differentiation ;
>>Long-term potentiation ;
>>Neurotrophin signaling pathway ;
>>Cholinergic synapse ;
>>Oxytocin signaling pathway ;
>>Aldosterone synthesis and secretion ;
>>Amphetamine addiction ;
>>Alcoholism ;
>>Glioma