The antiserum was produced against synthesized peptide derived from human iNOS around the phosphorylation site of Tyr151. AA range:117-166

Phospho-NOS2 (Y151) Polyclonal Antibody detects endogenous levels of NOS2 protein only when phosphorylated at Y151.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):QYyGS

NOS2, INOS

Nitric oxide synthase inducible

NOS2 ;
NOS2A ;
Nitric oxide synthase ;
inducible ;
Hepatocyte NOS ;
HEP-NOS ;
Inducible NO synthase ;
Inducible NOS ;
iNOS ;
NOS type II ;
Peptidyl-cysteine S-nitrosylase NOS2

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

Catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,Function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Binds SLC9A3R1.,tissue specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.,

Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .

>>Arginine biosynthesis ;
>>Arginine and proline metabolism ;
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>HIF-1 signaling pathway ;
>>Peroxisome ;
>>Apelin signaling pathway ;
>>Relaxin signaling pathway ;
>>Alzheimer disease ;
>>Amyotrophic lateral sclerosis ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Pertussis ;
>>Leishmaniasis ;
>>Chagas disease ;
>>Toxoplasmosis ;
>>Amoebiasis ;
>>Tuberculosis ;
>>Pathways in cancer ;
>>Small cell lung cancer