WB 1:10000-50000; ELISA 1:20000-50000; IP 1:50-1:200; IF 1:1000 -3000
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Specificity
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Purification
Protein A purified
Purity
>95% SDS-PAGE
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Modification
Unmodified
Clonality
Monoclonal
Clone Number
12M11
Isotype
VH IgG1 and VL Kappa
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Antigen&Target Information
Immunogen:
Synthetic Peptide of Flag-Tag
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Specificity:
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
show all
Gene Name:
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
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Background:
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.