Contact Us

408-747-0189
1-877-594-3616

Quick order
O OE R ip

DDDDK-Tag(binds to flag sequnence) (12M11) Mouse mAb

-YM3808

hot
Main Information
Target

Flag-tag

Host Species

Mouse

Reactivity

Species independent

Applications

WB, ELISA, IP, IF, CoIP

Conjugate/Modification


Unmodified

Detailed Information
Recommended Dilution Ratio
WB 1:10000-50000; ELISA 1:20000-50000; IP 1:50-1:200; IF 1:1000 -3000
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Specificity
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Purification
Protein A purified
Purity
>95% SDS-PAGE
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Modification
Unmodified
Clonality
Monoclonal
Clone Number
12M11
Isotype
VH IgG1 and VL Kappa
Related Products
Secondary Antibodies
Goat Anti Mouse IgG(H+L) (HRP)
RS0001

More→

Secondary Antibodies
Goat Anti Rabbit IgG(H+L) (HRP)
RS0002

More→

Primary Antibodies
β-actin (PTR2364) Mouse mAb
YM3028

More→

Primary Antibodies
GAPDH (PTR2304) Mouse mAb
YM3029

More→

Antigen&Target Information
Immunogen:
Synthetic Peptide of Flag-Tag
show all
Specificity:
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
show all
Gene Name:
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
show all
Background:
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
show all

Toggle night Mode

{{pinfoXq.title || ''}}

Catalog: {{pinfoXq.catalog || ''}}
Filter:

All

{{item.name}}

{{pinfo.title}}
-{{pinfo.catalog}}

Filter:

{{item.descr}}

Main Information
Target
{{pinfo.target}}
Reactivity
{{pinfo.react}}
Applications
{{pinfo.applicat}}
Conjugate/Modification
{{pinfo.coupling}}/{{pinfo.modific}}
MW (kDa)
{{pinfo.mwcalc}}
Host Species
{{pinfo.hostspec}}
Isotype
{{pinfo.isotype}}
Learn more
Product {{index}}/{{pcount}}
Prev
Next

{{pvTitle}}

Scroll wheel zooms the picture
{{pvDescr}}