Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:WB 1:1000-2000; IHC 1:50-300.
Formulation
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Specificity
Cyclophilin B Monoclonal Antibody(2B10) HRP Conjugated, specially designed for your Western blot analysis.
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Concentration
1mg/ml
MW(Calculated)
24kD
Conjugate
HRP
Clonality
Monoclonal
Clone Number
2B10
Isotype
IgG
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Antigen&Target Information
Specificity:
Cyclophilin B Monoclonal Antibody(2B10) HRP Conjugated, specially designed for your Western blot analysis.
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Gene Name:
PPIB
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Protein Name:
Peptidyl-prolyl cis-trans isomerase B (PPIase B) (CYP-S1) (Cyclophilin B) (Rotamase B) (S-cyclophilin) (SCYLP)
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009],
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Function:
Catalytic activity:Peptidylproline (omega=180) = peptidylproline (omega=0).,Caution:It is uncertain whether Met-1 or Met-9 is the initiator.,enzyme regulation:Cyclosporin A (CsA) inhibits CYPB.,Function:PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.,similarity:Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.,similarity:Contains 1 PPIase cyclophilin-type domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
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Cellular Localization:
Virion . (Microbial infection).; Endoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .