ZAP70

Tyrosine-protein kinase ZAP-70 ;
70 kDa zeta-chain associated protein ;
Syk-related tyrosine kinase ;

catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. ,disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. ,domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z. ,function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction , isoform 2 does not. ,online information:ZAP70 mutation db ,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. ,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 2 SH2 domains. ,subcellular location:After antigen stimulation , isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. ,subunit:Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity) . Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity) . Interacts with FCRL3. ,tissue specificity:Expressed in T- and natural killer cells. ,

cell activation , activation of immune response , immune response-activating cell surface receptor signaling pathway ,immune system development , leukocyte differentiation , positive regulation of immune system process , regulation of leukocyte activation , positive regulation of leukocyte activation , immune response-activating signal transduction ,immune response-regulating signal transduction , immune response-regulating cell surface receptor signaling pathway ,protein amino acid phosphorylation , phosphorus metabolic process , phosphate metabolic process , immune response ,cell surface receptor linked signal transduction , intracellular signaling cascade , protein kinase cascade , positive regulation of cell proliferation , positive regulation of signal transduction , positive regulation of cell communication ,phosphorylation , peptidyl-tyrosine phosphorylation , peptidyl-tyrosine modification , hemopoiesis , lymphocyte differentiation , T cell differentiation , regulation of mononuclear cell proliferation , positive regulation of mononuclear cell proliferation , T cell differentiation in the thymus , positive regulation of T cell proliferation , T cell activation , regulation of cell proliferation , regulation of T cell proliferation , beta selection , positive T cell selection , negative T cell selection , T cell selection , positive thymic T cell selection , negative thymic T cell selection , thymic T cell selection , leukocyte activation , regulation of T cell differentiation , positive regulation of T cell differentiation , positive regulation of cell differentiation , regulation of lymphocyte differentiation , positive regulation of lymphocyte differentiation , alpha-beta T cell activation , alpha-beta T cell differentiation , regulation of alpha-beta T cell activation , positive regulation of alpha-beta T cell activation , regulation of alpha-beta T cell differentiation , positive regulation of alpha-beta T cell differentiation , regulation of alpha-beta T cell proliferation , positive regulation of alpha-beta T cell proliferation ,lymphocyte activation , protein amino acid autophosphorylation , hemopoietic or lymphoid organ development , positive regulation of response to stimulus , regulation of lymphocyte proliferation , positive regulation of lymphocyte proliferation , positive regulation of immune response , regulation of calcium-mediated signaling , positive regulation of calcium-mediated signaling , antigen receptor-mediated signaling pathway , T cell receptor signaling pathway ,regulation of T cell activation , regulation of cell activation , positive regulation of cell activation , positive regulation of T cell activation , positive regulation of developmental process , regulation of lymphocyte activation , positive regulation of lymphocyte activation , regulation of leukocyte proliferation , positive regulation of leukocyte proliferation ,

Cytoplasm . Cell membrane ; Peripheral membrane protein . In quiescent T-lymphocytes , it is cytoplasmic. Upon TCR activation , it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) . .