PDE4B/PDE4C/PDE4D

cAMP-specific 3',5'-cyclic phosphodiesterase 4D ;
DPDE3 ;
PDE43 ;

catalytic activity:Adenosine 3' ,5'-cyclic phosphate + H (2) O = adenosine 5'-phosphate. ,cofactor:Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions , while site 2 has a preference for magnesium and/or manganese ions. ,disease:Genetic variations in PDE4D might be associated with susceptibility to stroke type 1 (STRK1) [MIM:606799]. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor , sensory and/or cognitive function. PubMed:17006457 states that association with stroke has to be considered with caution. ,enzyme regulation:Inhibited by rolipram. Activated by phosphatidic acid. ,function:Regulates the levels of cAMP in the cell. ,pathway:Purine metabolism; cAMP degradation; AMP from cAMP: step 1/1. ,PTM:Isoform 2 and isoform 11 are activated by phosphorylation (in vitro) , but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49 , Ser-51 , Ser-55 and Ser-59. ,similarity:Belongs to the cyclic nucleotide phosphodiesterase family. ,subcellular location:Found in the soluble fraction , associated with membranes , and associated with the cytoskeleton and the centrosome. ,subunit:Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A , PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity) . Isoform 5 binds GNB2L1 via its unique N-terminus. Binds ARRB2. ,tissue specificity:Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain , placenta , lung and kidney. Isoform 11 is detected in heart and skeletal muscle. ,

muscle system process , purine nucleotide metabolic process , purine nucleotide catabolic process , cAMP catabolic process , muscle contraction , smooth muscle contraction , nucleoside monophosphate metabolic process , nucleoside monophosphate catabolic process , nucleotide catabolic process , cyclic nucleotide metabolic process , cyclic nucleotide catabolic process , nucleobase , nucleoside , nucleotide and nucleic acid catabolic process , nucleobase , nucleoside and nucleotide catabolic process , nitrogen compound catabolic process , cAMP metabolic process , heterocycle catabolic process ,

Apical cell membrane . Cytoplasm . Membrane . Cytoplasm , cytoskeleton . Cytoplasm , cytoskeleton , microtubule organizing center , centrosome . Found in the soluble fraction , associated with membranes , and associated with the cytoskeleton and the centrosome (By similarity) . Colocalized with SHANK2 to the apical membrane of colonic crypt cells. .