Detects endogenous levels of p46 and p54 JNK when phosphorylated at Thr183 and Tyr185.

MAPK8/9/10

catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. ,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases , MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases , such as DUSP1. ,function:JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun , whereas alpha-1 , alpha-2 , and beta-2 have a similar low level of binding to both c-Jun or ATF2. However , there is no correlation between binding and phosphorylation , which is achieved at about the same efficiency by all isoforms. ,function:Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors , primarily components of AP-1 such as JUN , JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells , JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity) . Phosphorylates heat shock factor protein 4 (HSF4) . ,online information:C-Jun N-terminal kinases entry ,PTM:Dually phosphorylated on Thr-183 and Tyr-185 , which activates the enzyme. ,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,subunit:Binds to at least four scaffolding proteins , MAPK8IP1/JIP-1 , MAPK8IP2/JIP-2 , MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF (By similarity) . Interacts with NFATC4. ,

MAPKKK cascade , skeletal system development , ossification , protein amino acid phosphorylation , phosphorus metabolic process , phosphate metabolic process , apoptosis , induction of apoptosis , cell motion , intracellular signaling cascade , protein kinase cascade , JNK cascade , JUN phosphorylation , cell death , induction of apoptosis by extracellular signals , activation of pro-apoptotic gene products , response to radiation , response to UV , response to light stimulus ,response to abiotic stimulus , response to inorganic substance , response to metal ion , regulation of cell death , positive regulation of cell death , programmed cell death , induction of programmed cell death , death , phosphorylation , peptidyl-threonine phosphorylation , peptidyl-threonine modification , stress-activated protein kinase signaling pathway ,induction of programmed cell death in response to chemical stimulus , induction of apoptosis in response to chemical stimulus , cellular response to stress , regulation of apoptosis , positive regulation of apoptosis , negative regulation of apoptosis , regulation of programmed cell death , positive regulation of programmed cell death , negative regulation of programmed cell death , response to cadmium ion , bone development , negative regulation of cell death ,

Cytoplasm . Nucleus . Cell junction , synapse . In the cortical neurons , predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity) . Colocalizes with POU5F1 in the nucleus. .