DAXX

Death domain-associated protein 6 ;
Daxx ;
hDaxx ;
ETS1-associated protein 1 ;
EAP1 ;
Fas death domain-associated protein ;

function:Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast , in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML , respectively. ,induction:Upon mitogenic stimulation by concanavalin A. ,PTM:Phosphorylated upon DNA damage , probably by ATM or ATR. Phosphorylated by HIPK1 upon glucose deprivation. ,PTM:Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. ,PTM:Sumoylated. ,similarity:Belongs to the DAXX family. ,subcellular location:Dispersed throughout the nucleoplasm , in PML/POD/ND10 nuclear bodies , and in nucleoli. Colocalizes with a subset of interphase centromeres , but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. ,subunit:Homomultimer. Binds to the TNFRSF6 death domain via its C-terminus and to PAX5. Binds to SLC2A4/GLUT4 , MAP3K5 , TGFBR2 , phosphorylated dimeric HSPB1/HSP27 , CENPC1 , ETS1 , sumoylated PML , UBE2I and MCRS1. Is part of a complex containing PAX5 and CREBBP. Interacts with HIPK2 and HIPK3 via its N-terminus. Interacts with HIPK1 , which induces translocation from PML/POD/ND10 nuclear bodies to chromatin and enhances association with HDAC1 (By similarity) . The non-phosphorylated form binds to PAX3 , PAX7 , DEK , HDAC1 , HDAC2 , HDAC3 , acetylated histone H4 and histones H2A , H2B , H3 and H4. Interacts with SPOP. Part of a complex consisting of DAXX , CUL3 and SPOP. Interacts with CBP; the interaction is dependent the sumoylation of CBP and suppresses CBP transcriptional activity via recruitment of HDAC2 (By similarity) . Interacts with HCMV tegument phosphoprotein pp71. ,tissue specificity:Ubiquitous. ,

MAPKKK cascade , activation of MAPK activity , cytokinesis after mitosis , cytokinesis , regulation of protein amino acid phosphorylation , transcription , regulation of transcription , DNA-dependent , protein amino acid phosphorylation ,phosphorus metabolic process , phosphate metabolic process , apoptosis , induction of apoptosis , cell cycle , intracellular signaling cascade , protein kinase cascade , JNK cascade , activation of JUN kinase activity , cell death , induction of apoptosis by extracellular signals , negative regulation of biosynthetic process , negative regulation of macromolecule biosynthetic process , negative regulation of macromolecule metabolic process , regulation of protein kinase cascade ,negative regulation of gene expression , regulation of cell death , positive regulation of cell death , programmed cell death , induction of programmed cell death , death , phosphorylation , negative regulation of transcription , regulation of phosphate metabolic process , cell cycle process , steroid hormone receptor signaling pathway , androgen receptor signaling pathway , intracellular receptor-mediated signaling pathway , stress-activated protein kinase signaling pathway , negative regulation of cellular biosynthetic process , regulation of protein modification process , regulation of cellular protein metabolic process , cytokinesis during cell cycle , cellular response to stress , positive regulation of kinase activity , regulation of phosphorylation , regulation of apoptosis , positive regulation of apoptosis , regulation of programmed cell death , positive regulation of programmed cell death , positive regulation of catalytic activity ,regulation of MAP kinase activity , positive regulation of MAP kinase activity , regulation of MAPKKK cascade , regulation of JUN kinase activity , positive regulation of JUN kinase activity , regulation of kinase activity , positive regulation of molecular function , regulation of transcription , regulation of protein kinase activity , positive regulation of protein kinase activity , negative regulation of transcription , DNA-dependent , negative regulation of nucleobase , nucleoside , nucleotide and nucleic acid metabolic process , regulation of JNK cascade , negative regulation of nitrogen compound metabolic process , regulation of phosphorus metabolic process , regulation of RNA metabolic process , negative regulation of RNA metabolic process , cell division , regulation of transferase activity , positive regulation of transferase activity , regulation of stress-activated protein kinase signaling pathway , regulation of cellular response to stress ,

Cytoplasm . Nucleus , nucleoplasm . Nucleus , PML body . Nucleus , nucleolus . Chromosome , centromere . Dispersed throughout the nucleoplasm , in PML/POD/ND10 nuclear bodies , and in nucleoli (Probable) . Colocalizes with histone H3.3 , ATRX , HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102 , PubMed:14990586 , PubMed:23222847 , PubMed:24200965) . Colocalizes with a subset of interphase centromeres , but is absent from mitotic centromeres (PubMed:9645950) . Detected in cytoplasmic punctate structures (PubMed:11842083) . Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034) . Colocalizes with RASSF1 in the nucleus (PubMed:18566590) . Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383) . .; [Isoform beta]: Nucleus . Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1. .; [Isoform gamma]: Nucleus . Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1. .