KSR1

Kinase suppressor of Ras 1

caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. ,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself , it has no demonstrated kinase activity. ,PTM:Phosphorylated on Ser-309 and , to a higher extent , on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells , phosphorylated KSR1 is cytoplasmic and in stimulated cells , dephosphorylated KSR1 is membrane-associated. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ,similarity:Contains 1 phorbol-ester/DAG-type zinc finger. ,similarity:Contains 1 protein kinase domain. ,subcellular location:In unstimulated cells , where the phosphorylated form is bound to a 14-3-3 protein , sequestration in the cytoplasm occurs. Following growth factor treatment , the protein is free for membrane translocation , and it moves from the cytoplasm to the cell periphery. ,subunit:Interacts with HSPCA/HSP90 , YWHAB/14-3-3 , CDC37 , MAP2K/MEK , MARK3 , PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK , in a Ras-dependent manner (By similarity) . The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. ,

protein amino acid phosphorylation , phosphorus metabolic process , phosphate metabolic process , intracellular signaling cascade , small GTPase mediated signal transduction , Ras protein signal transduction , phosphorylation ,

Cytoplasm . Membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cell projection , ruffle membrane . Endoplasmic reticulum membrane . In unstimulated cells , where the phosphorylated form is bound to a 14-3-3 protein , sequestration in the cytoplasm occurs. Following growth factor treatment , the protein is free for membrane translocation , and it moves from the cytoplasm to the cell periphery. .