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Catalog: YD0119
Size
Price
Status
Qty.
100μg
$280.00
3 weeks

0

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Main Information
Reactivity

Human

Applications

WB, SDS-PAGE

Detailed Information
Recommended Dilution Ratio
WB 1:500-2000
Formulation
Liquid in PBS
Source
E.coli
Purity
SDS-PAGE >90%
Storage
-20°C/6 month,-80°C for long storage
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Antigen&Target Information
Sequence:
Amino acid: 492-648, with his-MBP tag.
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Gene Name:
RAF1
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Protein Name:
c-Raf protein
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Other Name:
RAF proto-oncogene serine/threonine-protein kinase ;
Proto-oncogene c-RAF ;
cRaf ;
Raf-1 ;
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Database Link:
Organism Gene ID SwissProt
Human 5894; P04049;
Mouse Q99N57;
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,disease:Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.,disease:Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.,function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3; the interaction inhibits its pro-apoptotic activity. Interacts with YWHAZ (unphosphorylated at 'Thr-232').,tissue specificity:In skeletal muscle, isoform 1 is more abundant than isoform 2.,
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Function:
protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, apoptosis,cytoskeleton organization, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway, intracellular signaling cascade, small GTPase mediated signal transduction, Ras protein signal transduction, cell death, cell proliferation, programmed cell death, death, phosphorylation, nerve growth factor receptor signaling pathway,
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Cellular Localization:
Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
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Tissue Expression:
In skeletal muscle, isoform 1 is more abundant than isoform 2.
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Signaling Pathway
Cellular Processes >> Transport and catabolism >> Autophagy - animal Cellular Processes >> Cell growth and death >> Apoptosis Cellular Processes >> Cell growth and death >> Cellular senescence Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion Cellular Processes >> Cellular community - eukaryotes >> Gap junction Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton Organismal Systems >> Immune system >> Neutrophil extracellular trap formation Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity Organismal Systems >> Immune system >> T cell receptor signaling pathway Organismal Systems >> Immune system >> B cell receptor signaling pathway Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis Organismal Systems >> Immune system >> Chemokine signaling pathway Organismal Systems >> Endocrine system >> Insulin signaling pathway Organismal Systems >> Endocrine system >> GnRH signaling pathway Organismal Systems >> Endocrine system >> Estrogen signaling pathway Organismal Systems >> Endocrine system >> Prolactin signaling pathway Organismal Systems >> Endocrine system >> Oxytocin signaling pathway Organismal Systems >> Endocrine system >> Relaxin signaling pathway Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction Organismal Systems >> Nervous system >> Neurotrophin signaling pathway Human Diseases >> Cancer: overview >> Pathways in cancer Human Diseases >> Cancer: overview >> MicroRNAs in cancer Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer Human Diseases >> Cancer: specific types >> Colorectal cancer Human Diseases >> Cancer: specific types >> Pancreatic cancer Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma Human Diseases >> Cancer: specific types >> Gastric cancer Human Diseases >> Cancer: specific types >> Glioma Human Diseases >> Cancer: specific types >> Acute myeloid leukemia Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia Human Diseases >> Cancer: specific types >> Melanoma Human Diseases >> Cancer: specific types >> Renal cell carcinoma Human Diseases >> Cancer: specific types >> Bladder cancer Human Diseases >> Cancer: specific types >> Prostate cancer Human Diseases >> Cancer: specific types >> Endometrial cancer Human Diseases >> Cancer: specific types >> Breast cancer Human Diseases >> Cancer: specific types >> Non-small cell lung cancer Human Diseases >> Neurodegenerative disease >> Alzheimer disease Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases Environmental Information Processing >> Signal transduction >> MAPK signaling pathway Environmental Information Processing >> Signal transduction >> ErbB signaling pathway Environmental Information Processing >> Signal transduction >> Ras signaling pathway Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway Environmental Information Processing >> Signal transduction >> VEGF signaling pathway Environmental Information Processing >> Signal transduction >> Apelin signaling pathway Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway Environmental Information Processing >> Signal transduction >> FoxO signaling pathway Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway Environmental Information Processing >> Signal transduction >> cAMP signaling pathway Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Catalog: YD0119
Size
Price
Status
Qty.
100μg
$280.00
3 weeks

0

Add to cart

Collected

Collect

Customized Service

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