TNK2

Activated CDC42 kinase 1 ;
ACK-1 ;
EC 2.7.11.1 ;
Tyrosine kinase non-receptor protein 2 ;

catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Magnesium.,enzyme regulation:The SH3 domain appears to play an autoinhibitory role.,function:Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.,sequence caution:Unlikely isoform. Aberrant splice sites.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with CDC42. Interacts with activated CSPG4.,

regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, intracellular signaling cascade, small GTPase mediated signal transduction, positive regulation of phosphorus metabolic process, positive regulation of macromolecule metabolic process, phosphorylation, regulation of phosphate metabolic process,regulation of protein modification process, positive regulation of protein modification process, regulation of cellular protein metabolic process, positive regulation of cellular protein metabolic process, regulation of phosphorylation,positive regulation of phosphorylation, positive regulation of phosphate metabolic process, regulation of peptidyl-tyrosine phosphorylation, positive regulation of peptidyl-tyrosine phosphorylation, regulation of phosphorus metabolic process, positive regulation of protein metabolic process,

Cell membrane . Nucleus . Endosome . Cell junction, adherens junction . Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle . Membrane, clathrin-coated pit . Cytoplasm, perinuclear region . Cytoplasm, cytosol . The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity). Co-localizes with EGFR on endosomes (PubMed:20333297). Nuclear translocation is CDC42-dependent (By similarity). Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity). .

The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.