SGOL1

Shugoshin-like 1 ;
hSgo1 ;
Serologically defined breast cancer antigen NY-BR-85 ;

developmental stage:Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).,domain:The D-box (destruction box) mediates the interaction with APC proteins, and may act as a recognition signal for degradation via the ubiquitin-proteasome pathway.,function:Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase.,miscellaneous:Strongly overexpressed in 90% of breast cancers tested.,PTM:Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.,similarity:Belongs to the shugoshin family.,subcellular location:Localizes to the centromere throughout prophase until metaphase and disappears at anaphase. BUB1 is required for centromeric localization. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres.,subunit:Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E.,tissue specificity:Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta.,

M phase of mitotic cell cycle, mitotic cell cycle, M phase, nuclear division, cell cycle, chromosome segregation, mitosis,meiosis, cell cycle process, cell cycle phase, meiotic chromosome segregation, organelle fission, cell division, meiotic cell cycle, M phase of meiotic cell cycle,

Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase (PubMed:16541025). Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A (PubMed:16580887)(PubMed:16541025)(PubMed:15604152). Colocalizes with NEK2 at the kinetochore (PubMed:17621308). Colocalizes with and SS18L1 at the kinetochore (PubMed:16582621). Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore (PubMed:17617734). Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis (PubMed:16582621). Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent (PubMed:16582621). Isoform 3 does not localize to kinetochores during any stages of the cell cycle (PubMed:16582621). .

Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.