Contact Us

408-747-0189
1-877-594-3616

Quick order

FANCD2 (Phospho Ser222) Cell-Based Colorimetric ELISA Kit

-KA1250C

Catalog: KA1250C
Size
Price
Status
Qty.
96well
$470.00
In stock

0

Add to cart

Collected

Collect

Customized Service
Main Information
Reactivity

Human, Mouse, Rat

Applications

ELISA

Conjugate/Modification


Phospho

Detailed Information
Storage
2-8°C/6 months
Modification
Phospho
Detection Method
Colorimetric
Related Products
Primary Antibodies
FANCD2 Rabbit pAb
YT1675

More→

Primary Antibodies
FANCD2 (Phospho Ser222) Rabbit pAb
YP0630

More→

ELISA Kits
Total FANCD2 Cell-Based Colorimetric ELISA Kit
KA4095C

More→

ELISA Kits
FANCD2 (Phospho Ser222) Cell-Based Colorimetric ELISA Kit
KA1250C

More→

Antigen&Target Information
Gene Name:
FANCD2
show all
Other Name:
Fanconi anemia group D2 protein ;
Protein FACD2 ;
show all
Database Link:
Organism Gene ID SwissProt
Human 2177; Q9BXW9;
Mouse Q80V62;
Rat Q6IV68;
Background:
developmental stage:Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).,disease:Defects in FANCD2 are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.,domain:The C-terminal 24 residues of isoform 2 are required for its function.,function:Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.,PTM:Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1 and BRCA2, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.,PTM:Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.,subcellular location:Concentrates in nuclear foci during S phase and upon genotoxic stress.,subunit:Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1, BRCA2 and BLM.,tissue specificity:Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.,
show all
Function:
M phase, DNA metabolic process, DNA repair, response to DNA damage stimulus, cell cycle, meiosis, meiosis I,synapsis, gamete generation, response to radiation, response to abiotic stimulus, response to ionizing radiation,response to gamma radiation, sexual reproduction, cell cycle process, cell cycle phase, multicellular organism reproduction, cellular response to stress, reproductive process in a multicellular organism, chromosome organization,meiotic cell cycle, M phase of meiotic cell cycle, chromosome organization involved in meiosis,
show all
Cellular Localization:
Nucleus . Concentrates in nuclear foci during S phase and upon genotoxic stress. At the onset of mitosis, excluded from chromosomes and diffuses into the cytoplasm, returning to the nucleus at the end of cell division. Observed in a few spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites and could be sites of replication fork stalling. The foci are frequently interlinked through BLM-associated ultra-fine DNA bridges. Following aphidicolin treatment, targets chromatid gaps and breaks.
show all
Tissue Expression:
Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
show all
Catalog: KA1250C
Size
Price
Status
Qty.
96well
$470.00
In stock

0

Add to cart

Collected

Collect

Customized Service

Toggle night Mode

{{pinfoXq.title || ''}}

Catalog: {{pinfoXq.catalog || ''}}
Filter:

All

{{item.name}}

{{pinfo.title}}
-{{pinfo.catalog}}

Filter:

{{item.descr}}

Main Information
Target
{{pinfo.target}}
Reactivity
{{pinfo.react}}
Applications
{{pinfo.applicat}}
Conjugate/Modification
{{pinfo.coupling}}/{{pinfo.modific}}
MW (kDa)
{{pinfo.mwcalc}}
Host Species
{{pinfo.hostspec}}
Isotype
{{pinfo.isotype}}
Learn more
Product {{index}}/{{pcount}}
Prev
Next

{{pvTitle}}

Scroll wheel zooms the picture
{{pvDescr}}