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WNK1 (Phospho Thr58) Cell-Based Colorimetric ELISA Kit

-KA1226C

Catalog: KA1226C
Size
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96well
$470.00
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Main Information
Reactivity

Human, Mouse, Rat

Applications

ELISA

Conjugate/Modification


Phospho

Detailed Information
Storage
2-8°C/6 months
Modification
Phospho
Detection Method
Colorimetric
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Antigen&Target Information
Gene Name:
WNK1
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Other Name:
Serine/threonine-protein kinase WNK1 ;
Erythrocyte 65 kDa protein ;
p65 ;
Kinase deficient protein ;
Protein kinase lysine-deficient 1 ;
Protein kinase with no lysine 1 ;
hWNK1 ;
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Database Link:
Organism Gene ID SwissProt
Human 65125; Q9H4A3;
Mouse P83741;
Rat Q9JIH7;
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Cys-250 is present instead of the conserved Lys which is expected to be an active site residue. Lys-233 appears to fulfill the required catalytic function.,caution:PubMed:2507249 describes a peptide sequence containing a GlcNAc glycosylated Ser in position 164 while it is an Arg residue according to others.,cofactor:Magnesium.,disease:Defects in WNK1 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.,enzyme regulation:By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity.,function:Controls sodium and chloride ion transport by inhibiting the activity of WNK4, potentially by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization.,PTM:O-glycosylated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with SYT2.,tissue specificity:Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle.,
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Function:
protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, ion transport,intracellular signaling cascade, protein kinase cascade, phosphorylation,
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Cellular Localization:
Cytoplasm .
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Tissue Expression:
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
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Catalog: KA1226C
Size
Price
Status
Qty.
96well
$470.00
In stock

0

Add to cart

Collected

Collect

Customized Service

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