Synthesized peptide derived from human C-RAF (Phospho Tyr340)

This antibody detects endogenous levels of C-RAF (Phospho Tyr340) Rabbit pAb at Human, Mouse,Rat

RAF1 RAF

RAF proto-oncogene serine/threonine-protein kinase (Proto-oncogene c-RAF) (cRaf) (Raf-1)

RAF proto-oncogene serine/threonine-protein kinase ;
Proto-oncogene c-RAF ;
cRaf ;
Raf-1 ;

Raf-1 proto-oncogene , serine/threonine kinase (RAF1) Homo sapiens This gene is the cellular homolog of viral raf gene (v-raf) . The encoded protein is a MAP kinase kinase kinase (MAP3K) , which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated , the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 , which in turn phosphorylate to activate the serine/threonine specific protein kinases , ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle , apoptosis , cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq , Jul 2008] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Binds 2 zinc ions per subunit. ,Disease:Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines , electrocardiographic conduction abnormalities , ocular hypertelorism , pulmonic stenosis , abnormalities of genitalia , retardation of growth , and deafness. ,Disease:Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features , short stature , hypertelorism , cardiac anomalies , deafness , motor delay , and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome , with an estimated incidence of 1 in 1000-2500 live births. ,Function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. ,PTM:Phosphorylated upon DNA damage , probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. ,similarity:Contains 1 phorbol-ester/DAG-type zinc finger. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 1 RBD (Ras-binding) domain. ,subunit:Interacts with Ras proteins; the interaction is antagonized by RIN1. Weakly interacts with RIT1 (By similarity) . Interacts with STK3; the interaction inhibits its pro-apoptotic activity. Interacts with YWHAZ (unphosphorylated at 'Thr-232') . ,tissue specificity:In skeletal muscle , isoform 1 is more abundant than isoform 2. ,

Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.

>>EGFR tyrosine kinase inhibitor resistance ;
>>Endocrine resistance ;
>>MAPK signaling pathway ;
>>ErbB signaling pathway ;
>>Ras signaling pathway ;
>>Rap1 signaling pathway ;
>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>Chemokine signaling pathway ;
>>FoxO signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>Autophagy - animal ;
>>mTOR signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>Apoptosis ;
>>Cellular senescence ;
>>Vascular smooth muscle contraction ;
>>Axon guidance ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Focal adhesion ;
>>Gap junction ;
>>Signaling pathways regulating pluripotency of stem cells ;
>>Neutrophil extracellular trap formation ;
>>C-type lectin receptor signaling pathway ;
>>JAK-STAT signaling pathway ;
>>Natural killer cell mediated cytotoxicity ;
>>T cell receptor signaling pathway ;
>>B cell receptor signaling pathway ;
>>Fc epsilon RI signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Long-term potentiation ;
>>Neurotrophin signaling pathway ;
>>Serotonergic synapse ;
>>Long-term depression ;
>>Regulation of actin cytoskeleton ;
>>Insulin signaling pathway ;
>>GnRH signaling pathway ;
>>Progesterone-mediated oocyte maturation ;
>>Estrogen signaling pathway ;
>>Melanogenesis ;
>>Prolactin signaling pathway ;
>>Thyroid hormone signaling pathway ;
>>Oxytocin signaling pathway ;
>>Relaxin signaling pathway ;
>>Parathyroid hormone synthesis , secretion and action ;
>>GnRH secretion ;
>>Growth hormone synthesis , secretion and action ;
>>Alzheimer disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Alcoholism ;
>>Salmonella infection ;
>>Tuberculosis ;
>>Hepatitis C ;
>>Hepatitis B ;
>>Human cytomegalovirus infection ;
>>Influenza A ;
>>Human papillomavirus infection ;
>>Kaposi sarcoma-associated herpesvirus infection ;
>>Human immunodeficiency virus 1 infection ;
>>Pathways in cancer ;
>>Proteoglycans in cancer ;
>>MicroRNAs in cancer ;
>>Chemical carcinogenesis - receptor activation ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Colorectal cancer ;
>>Renal cell carcinoma ;
>>Pancreatic cancer ;
>>Endometrial cancer ;
>>Glioma ;
>>Prostate cancer ;
>>Melanoma ;
>>Bladder cancer ;
>>Chronic myeloid leukemia ;
>>Acute myeloid leukemia ;
>>Non-small cell lung cancer ;
>>Breast cancer ;
>>Hepatocellular carcinoma ;
>>Gastric cancer ;
>>Central carbon metabolism in cancer ;
>>Choline metabolism in cancer ;
>>PD-L1 expression and PD-1 checkpoint pathway in cancer