Synthesized phosho peptide around human Ezh2 (Thr311)

This antibody detects endogenous levels of Human Ezh2 (phospho-Thr311)

EZH2 KMT6

EZH2 (Thr311)

Histone-lysine N-methyltransferase EZH2 ;
ENX-1 ;
Enhancer of zeste homolog 2 ;
Lysine N-methyltransferase 6 ;

enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) Homo sapiens This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes , which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein , the VAV1 oncoprotein , and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq , Feb 2011] ,

Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N (6) -methyl-L-lysine. ,Caution:Two variants of the PRC2 complex have been described , termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms , although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (PubMed:15099518 and PubMed:15684044) , although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 (PubMed:16431907) . ,developmental stage:Expression decreases during senescence of embryonic fibroblasts (HEFs) . Expression peaks at the G1/S phase boundary. ,Function:Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex , which methylates 'Lys-9' and 'Lys-27' of histone H3 , leading to transcriptional repression of the affected target gene. Able to mono- , di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1 , H3K27me2 and H3K27me3 , respectively. Compared to EZH2-containing complexes , it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3 , which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases , thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8 , HOXA9 , MYT1 and CDKN2A. ,induction:Expression is induced by E2F1 , E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV- , IR- and bleomycin-induced DNA damage and by activation of TP53/p53. ,PTM:Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. ,similarity:Belongs to the histone-lysine methyltransferase family. EZ subfamily. ,similarity:Contains 1 SET domain. ,subunit:Binds ATRX via the SET domain (Probable) . Component of the PRC2/EED-EZH2 complex , which includes EED , EZH2 , SUZ12 , RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED , EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1 , DNMT3A , DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. ,tissue specificity:Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast , colon , larynx , lymphoma and testis. ,

Nucleus . Localizes to the inactive X chromosome in trophoblast stem cells. .

>>Lysine degradation ;
>>Metabolic pathways ;
>>MicroRNAs in cancer