Synthesized phosho peptide around human CAD (Ser1859)

This antibody detects endogenous levels of Human Mouse Rat CAD (phospho-Ser1859)

CAD

CAD (Ser1859)

CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase ;
Aspartate carbamoyltransferase ;
Dihydroorotase ;
]

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II) , aspartate transcarbamoylase , and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade , which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq , Apr 2015] ,

Catalytic activity: (S) -dihydroorotate + H (2) O = N-carbamoyl-L-aspartate. ,Catalytic activity:2 ATP + L-glutamine + HCO (3) (-) + H (2) O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. ,Catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. ,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) . ,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction. ,Function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase , CPSase , ATCase and DHOase) . ,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5) . ,online information:Aspartate carbamoyltransferase entry ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 1/6. ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 2/6. ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 3/6. ,similarity:Belongs to the ATCase/OTCase family. ,similarity:Contains 1 glutamine amidotransferase type-1 domain. ,similarity:Contains 2 ATP-grasp domains. ,similarity:In the central section; belongs to the DHOase family. ,subunit:Homohexamer. ,

Cytoplasm . Nucleus . Cytosolic and unphosphorylated in resting cells , translocates to the nucleus in response to EGF stimulation , nuclear import promotes optimal cell growth.

>>Pyrimidine metabolism ;
>>Alanine , aspartate and glutamate metabolism ;
>>Metabolic pathways ;
>>Biosynthesis of cofactors