Catalog: YP1819
Size
Price
Status
Qty.
200μL
$936.00
In stock
0
100μL
$560.00
In stock
0
50μL
$300.00
In stock
0
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Collected
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Main Information
Target
MYPT1 Phospho Ser668/618
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
MW
130kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-2000; IHC 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Specificity
This antibody detects endogenous levels of MYPT1 only when phosphorylated at Human:S668/S618, Mouse:S666/S617, Rat:S669..The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RRsYL
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
130kD
Modification
Phospho
Clonality
Polyclonal
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Antigen&Target Information
Immunogen:
Synthesized peptide derived from human MYPT1/2 (Phospho Ser668/Ser618)
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Specificity:
This antibody detects endogenous levels of MYPT1 only when phosphorylated at Human:S668/S618, Mouse:S666/S617, Rat:S669..The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RRsYL
show all
Gene Name:
PPP1R12A MBS MYPT1 MYPT2
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Protein Name:
Protein phosphatase 1 regulatory subunit 12A (Myosin phosphatase-targeting subunit 1) (Myosin phosphatase target subunit 1) (Protein phosphatase myosin-binding subunit)
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Other Name:
Protein phosphatase 1 regulatory subunit 12A ;
Myosin phosphatase-targeting subunit 1 ;
Myosin phosphatase target subunit 1 ;
Protein phosphatase myosin-binding subunit ;
Myosin phosphatase-targeting subunit 1 ;
Myosin phosphatase target subunit 1 ;
Protein phosphatase myosin-binding subunit ;
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Database Link:
Background:
protein phosphatase 1 regulatory subunit 12A(PPP1R12A) Homo sapiens Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph
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Function:
Function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,
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Cellular Localization:
Cytoplasm . Cytoplasm, cytoskeleton, stress fiber . Also along actomyosin filaments. .
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Tissue Expression:
Research Areas:
>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Focal adhesion ;
>>Platelet activation ;
>>Regulation of actin cytoskeleton ;
>>Oxytocin signaling pathway ;
>>Proteoglycans in cancer
>>cAMP signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Focal adhesion ;
>>Platelet activation ;
>>Regulation of actin cytoskeleton ;
>>Oxytocin signaling pathway ;
>>Proteoglycans in cancer
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Signaling Pathway
Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion
Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton
Organismal Systems >> Immune system >> Platelet activation
Organismal Systems >> Endocrine system >> Oxytocin signaling pathway
Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction
Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Reference Citation({{totalcount}})
Catalog: YP1819
Size
Price
Status
Qty.
200μL
$936.00
In stock
0
100μL
$560.00
In stock
0
50μL
$300.00
In stock
0
Add to cart
Collected
Collect
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