Catalog: YP1683
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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Main Information
Target
SPHK1 Phospho Ser225
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB
MW
42kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-2000
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PAsPV
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
42kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
Synthesized peptide derived from human SPHK1 (Phospho-Ser225)
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Specificity:
This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PAsPV
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Gene Name:
SPHK1 SPHK SPK
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Protein Name:
Sphingosine kinase 1
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Other Name:
Sphingosine kinase 1 ;
SK 1 ;
SPK 1 ;
EC 2.7.1.91 ;
SK 1 ;
SPK 1 ;
EC 2.7.1.91 ;
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Database Link:
Background:
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
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Function:
Catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,Catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,Function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.,similarity:Contains 1 DAGKc domain.,subunit:Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP.,tissue specificity:Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.,
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Cellular Localization:
Cytoplasm . Nucleus . Cell membrane . Endosome membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse . Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). .
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Tissue Expression:
Research Areas:
>>Sphingolipid metabolism ;
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Tuberculosis
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Tuberculosis
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Signaling Pathway
Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis
Environmental Information Processing >> Signal transduction >> VEGF signaling pathway
Environmental Information Processing >> Signal transduction >> Apelin signaling pathway
Environmental Information Processing >> Signal transduction >> Calcium signaling pathway
Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway
Reference Citation({{totalcount}})
Catalog: YP1683
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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