Catalog: YP1181
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Collected
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Main Information
Target
Hck Phospho Tyr410
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, ELISA
MW
60kD (Calculated)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Hck (Y410) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y410.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):NEyTA
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
60kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human HCK around the phosphorylation site of Tyr410. AA range:381-430
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Specificity:
Phospho-Hck (Y410) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y410.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):NEyTA
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Gene Name:
HCK
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Protein Name:
Tyrosine-protein kinase HCK
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Other Name:
HCK ;
Tyrosine-protein kinase HCK ;
Hematopoietic cell kinase ;
Hemopoietic cell kinase ;
p59-HCK/p60-HCK ;
p59Hck ;
p61Hck
Tyrosine-protein kinase HCK ;
Hematopoietic cell kinase ;
Hemopoietic cell kinase ;
p59-HCK/p60-HCK ;
p59Hck ;
p61Hck
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Database Link:
Background:
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],
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Function:
Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:The SH3 domain mediates binding to HIV-1 Nef.,Function:May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.,PTM:Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:May interact (via SH3 domain) with HIV-1 Nef and Vif. This interaction would stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein.,tissue specificity:Expressed predominantly in cells of the myeloid and B-lymphoid lineages.,
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Cellular Localization:
[Isoform 1]: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.; [Isoform 2]: Cell membrane ; Lipid-anchor . Membrane, caveola ; Lipid-anchor . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Golgi apparatus . Cytoplasmic vesicle . Lysosome . Nucleus . 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.; Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.
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Tissue Expression:
Research Areas:
>>Chemokine signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Kaposi sarcoma-associated herpesvirus infection
>>Fc gamma R-mediated phagocytosis ;
>>Kaposi sarcoma-associated herpesvirus infection
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP1181
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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