Catalog: YP1108
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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Main Information
Target
SphK2 Phospho Thr614
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, WB, ELISA
MW
69kD (Calculated)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-SphK2 (T614) Polyclonal Antibody detects endogenous levels of SphK2 protein only when phosphorylated at T614.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PLtPR
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
69kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629
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Specificity:
Phospho-SphK2 (T614) Polyclonal Antibody detects endogenous levels of SphK2 protein only when phosphorylated at T614.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PLtPR
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Gene Name:
SPHK2
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Protein Name:
Sphingosine kinase 2
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Other Name:
SPHK2 ;
Sphingosine kinase 2 ;
SK 2 ;
SPK 2
Sphingosine kinase 2 ;
SK 2 ;
SPK 2
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Background:
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],
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Function:
Alternative products:Experimental confirmation may be lacking for some isoforms,Catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,Catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,Function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine.,similarity:Contains 1 DAGKc domain.,
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Cellular Localization:
Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547). .; [Isoform 2]: Lysosome membrane .
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Tissue Expression:
Research Areas:
>>Sphingolipid metabolism ;
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Tuberculosis
>>Metabolic pathways ;
>>Calcium signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>VEGF signaling pathway ;
>>Apelin signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Tuberculosis
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Signaling Pathway
Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis
Environmental Information Processing >> Signal transduction >> VEGF signaling pathway
Environmental Information Processing >> Signal transduction >> Apelin signaling pathway
Environmental Information Processing >> Signal transduction >> Calcium signaling pathway
Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway
Reference Citation({{totalcount}})
Catalog: YP1108
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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