Catalog: YP0730
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Collected
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Main Information
Target
Cdc25B Phospho Ser353
Host Species
Rabbit
Reactivity
Human, Monkey
Applications
WB, IHC, IF, ELISA
MW
64kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Cdc25B (S353) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S353.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RRsVT
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
64kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser353. AA range:319-368
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Specificity:
Phospho-Cdc25B (S353) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S353.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RRsVT
show all
Gene Name:
CDC25B
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Protein Name:
M-phase inducer phosphatase 2
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Other Name:
CDC25B ;
CDC25HU2 ;
M-phase inducer phosphatase 2 ;
Dual specificity phosphatase Cdc25B
CDC25HU2 ;
M-phase inducer phosphatase 2 ;
Dual specificity phosphatase Cdc25B
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Background:
cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],
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Function:
Catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,enzyme regulation:Stimulated by B-type cyclins.,Function:Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.,PTM:Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,
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Cellular Localization:
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
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Tissue Expression:
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Research Areas:
>>MAPK signaling pathway ;
>>Cell cycle ;
>>Progesterone-mediated oocyte maturation ;
>>MicroRNAs in cancer
>>Cell cycle ;
>>Progesterone-mediated oocyte maturation ;
>>MicroRNAs in cancer
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Signaling Pathway
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Catalog: YP0730
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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