Catalog: YP0722
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
PTP1B Phospho Ser50
Host Species
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ELISA
MW
49kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DVsPF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
49kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
Related Products
Primary Antibodies
PTP1B Rabbit pAb
YT3900
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Primary Antibodies
PTP1B (Phospho Ser50) Rabbit pAb
YP0722
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ELISA Kits
Total PTP1B Cell-Based Colorimetric ELISA Kit
KA4257C
More→
ELISA Kits
PTP1B (Phospho Ser50) Cell-Based Colorimetric ELISA Kit
KA1416C
More→
Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human PTP1B around the phosphorylation site of Ser50. AA range:16-65
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Specificity:
Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DVsPF
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Gene Name:
PTPN1
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Protein Name:
Tyrosine-protein phosphatase non-receptor type 1
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Other Name:
PTPN1 ;
PTP1B ;
Tyrosine-protein phosphatase non-receptor type 1 ;
Protein-tyrosine phosphatase 1B ;
PTP-1B
PTP1B ;
Tyrosine-protein phosphatase non-receptor type 1 ;
Protein-tyrosine phosphatase 1B ;
PTP-1B
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Database Link:
Background:
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of
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Function:
Catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,Function:May play an important role in CKII- and p60c-src-induced signal transduction cascades.,PTM:Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,
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Cellular Localization:
Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Interacts with EPHA3 at the cell membrane.
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Tissue Expression:
Research Areas:
>>Adherens junction ;
>>Insulin signaling pathway ;
>>Insulin resistance ;
>>Chemical carcinogenesis - reactive oxygen species
>>Insulin signaling pathway ;
>>Insulin resistance ;
>>Chemical carcinogenesis - reactive oxygen species
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0722
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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